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IBI Scientific

Fast-Ion Plasmid Kits

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Ultra-pure and large-volume plasmid preparation

IBI’s MIDI and MAXI Plasmid Kits use pre-packed, ion-exchange resin columns to purify plasmid or cosmid DNA from bacterial cultures. In this process, the modified alkaline lysis method and RNase treatment are used for creating cleared cell lysate with minimal genomic DNA and RNA contaminants. Using a gravity-flow procedure, the plasmid DNA in crude lysate has been bound to the column. The contaminants can be washed off with a wash buffer. Finally, the purified plasmid DNA is eluted by a high salt buffer and then precipitated with isopropanol for desalting. The entire procedure can be completed in less than 2 hours; and the obtained high purity plasmid DNA is suitable for transfection, sequencing reactions, PCR, and in-vitro transcription.

Sample Size:MIDI - 50ml for high copy plasmid / 100ml for low copy plasmid
MAXI - 100ml for high copy plasmid / 250ml for low copy plasmid
Format:Ion-Exchange Resign Column
Operation:Gravity Flow
Binding Capacity:MIDI - 500µg
MAXI - 1mg
Expextant Yield:MIDI - up to 200µg of plasmid DNA
MAXI - 500µg to 1mg of plasmid DNA
Purity:Equal to that obtained by 2XCsCl-Gradient Centrifugation
Operation Time:120 minutes or less
Applications:Transfection, Sequencing, In-Vitro Transcription, Microinjection, Restriction Digestion

Kit Components

Plasmid Columns2 MIDI25 MIDI2 MAXI10 MAXI25 MAXI
PM1 Buffer10mL110mL25mL110mL275mL
PM2 Buffer10mL110mL25mL110mL275mL
PM3 Buffer10mL110mL25mL110mL275mL
PEQ Buffer12mL130mL25mL130mL275mL
PW Buffer30mL360mL65mL360mL790mL
PEL Buffer25mL220mL25mL130mL350mL
RNase A (50mg/mL)Added200µLAdded200µL550µL

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Product Detail
Thomas No.
Mfr. No.
Also known as: DP-7111
Midi Fast-Ion Plasmid Kit-25 Preps
list price/quantitytotal
Thomas No.
Mfr. No.
Maxi Fast-Ion Plasmid Sample Kit-2 Preps
list price/quantitytotal
Thomas No.
Mfr. No.
Also known as: DP-7121
Maxi Fast-Ion Plasmid Kit-10 Preps
list price/quantitytotal
Thomas No.
Mfr. No.
Maxi Fast-Ion Plasmid Kit-25 Preps
list price/quantitytotal
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