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MP Biomedicals

MPure™ Bacterial DNA Extraction Kit

80 out of 100

MPure™ Bacterial DNA Extraction Kit

MPure Bacterial DNA Extraction Kit is used with the MPure-12 Nucleic Acid Purification System for extraction and purification of genomic DNA from both Gram-positive and Gram-negative bacteria. Nucleic acids extracted and purified from MPure Bacterial DNA Extraction Kit can be used in a number of downstream application including: PCR, qPCR, Sequencing (NGS), Microarray, RFLP and Southern Blot Analysis.

Key Applications:Nucleic acids extracted and purified from MPure Bacterial DNA Extraction Kit can be used in a number of downstream application including: PCR, qPCR, Sequencing (NGS), Microarray, RFLP and Southern Blot Analysis.
Application Areas:Nucleic Acid Purification - DNA from Gram-positive and Gram-negative bacteria.
Research Areas:Molecular Biology, Nucleic Acid Purification
Product Type:Kits & Assays
Instrument or System Type:Nucleic Acid Purification
Kit or Assay Type::Reagent Kit for Nucleic Acid Purification
Components:Proteinase K solution = 40 µL,Lysis Buffer 2 = 720 µL,Binding Buffer 1 = 720 µL,Magnetic Bead Solution = 800 µL,Washing Buffer 1 = 1000 µL,Washing Buffer 2 = 1000 µL,Washing Buffer 3 = 1000 µL,Elution Buffer 1 = 1000 µL,Elution Buffer 2 = 1000 µL
Instrument Compatibility:Use with MPure-12 System
Material of Construction:Plastic, Aluminum Foil and Buffers
Sample Type:Bacterial cells
Analyte(s):Detected DNA
Sample Volume:200-400 µL
Capacity:Bacterial Pellet: 200-400 µL /Up to 109 bacteria (about OD600 = 3); Bacterial Colony: 200-400µL /1-3 colony; Tissue: 200-400µL /1-30 mg; Urine: 200-400µL /5-50 mL urine; cell-Free body fluids: 200-400µL cell-free body fluids; Liquid Transport media: 200-400µL liquid transport media
Liquid Transport media:200-400µL liquid transport media
Sample Throughput:1-12 samples and 48 Preps/Kit
Molecule Enriched:DNA
Materials Required (Not Supplied):Pipettes, Pipette Tips, Test Tubes, Gloves
Sensitivity:As little of 20 copies (about 102 copy/ml bacteria in the sample) spiked-in (about 5 copy in PCR reaction) bacteria can be detected.
Storage & Handling:Store at room temperature (15-25°C). Do not freeze the reagent cartridges. The Kits are stable for 12 months under these conditions. Store the purified DNA at 4°C (short-term) or aliquot and store at –70°C (long-term) before performing the downstream analysis.
Certifications:CE
Dispense Volume Range:200-400 µL

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Product Detail
Thomas No.
C837J30
Mfr. No.
117022600
Description
MPure™ Bacterial DNA Extraction 1 Kit
list price/quantitytotal
$0.00
$0.00 (0 Items)
Product C837J30 is restricted and can only be purchased by customers with a web profile linked to a Thomas Business Account - Click here to login or create your web profile. If you do not have a Thomas Business Account, please Contact Us and someone will respond with details on how to apply for a business account with us.

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  • Reviewed on Wednesday, September 6, 2017

    80 out of 100

    Comments
    perfect!
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