2X One-Step RT-qPCR Master Mix (No passive dye)
The Launchworks 2X One-Step RT-qPCR Master Mix is designed to offer sensitive and reproducible detection of RNA and/or DNA targets in a single multiplex RT-qPCR reaction. The 2X format provides simple reaction setup and robust performance across many sample types and instruments.
Key features: Ability to run multiplexed reactions, Robust sensitivity for high and low-copy targets, Compatible across a dynamic range of RT-qPCR equipment, Built in tolerance to common clinical sample inhibitors, Only three steps from sample prep (i.e. RNA extraction) to getting results from your RT-qPCR results: 1) reagent mixing 2) plate dispensing 3) RT-qPCR method running.
Broad Dynamic Range: A master mix must be reliable across a dynamic range of copy numbers for any desired target. This is especially true in a clinical setting where pathogens may be present at low titers. Every lot of this 2X One-Step RT-qPCR Master Mix has consistent performance across 6 magnitudes of SARS CoV-2 E-gene copies. The master mix is designed to use only a single assay protocol to test for either (or both) RNA and DNA and requires no passive dye.
High Sensitivity: Performance of LaunchWorks produced master mix is comparable to ThermoFisher’s 4X TaqPath 1-Step Multiplex Master Mix. Across 6 magnitudes of the SARS CoV-2 E-gene, tested using FAM probe detection, the LaunchWorks produced master mix amplifies within 3 Ct of the Thermo Fisher master mix. As the copy number of E-gene present in the assay decreases, the LaunchWorks master mix amplification curve plateau reads stronger than the Thermo Fisher master mix.
Broad Instrument Compatibility: The Launchworks 2X One-Step RT-qPCR Master Mix performs across a wide range of real-time PCR systems. These systems include (but are not limited to) Applied Biosystems, BioRad CFX, Roche Lightcycler PCR platforms and Analytik Jena QTower 3.
High-Quality Enzymes: The master mix is made up of 4 highly specific and active enzymes: Reverse Transcriptase (RT), RNAse Inhibitor (RI), Taq DNA Polymerase (Taq), Uracil-DNA Glycosylase (UDG).
Manufactured in an ISO 13485:2016 certified facility under cGMP conditions.