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2X One-Step RT-qPCR Univeral Master Mix no ROX

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2X One-Step RT-qPCR Master Mix (No passive dye)

The Launchworks 2X One-Step RT-qPCR Master Mix is  designed to offer sensitive and reproducible detection of RNA and/or DNA targets in a single multiplex RT-qPCR reaction. The 2X format provides simple reaction setup and robust performance across many sample types and instruments.

Key features:  Ability to run multiplexed reactions, Robust sensitivity for high and low-copy targets, Compatible across a dynamic range of RT-qPCR equipment, Built in tolerance to common clinical sample inhibitors, Only three steps from sample prep (i.e. RNA extraction) to getting results from your RT-qPCR results:  1) reagent mixing 2) plate dispensing 3) RT-qPCR method running.

Broad Dynamic Range:  A master mix must be reliable across a dynamic range of copy numbers for any desired target. This is especially true in a clinical setting where pathogens may be  present  at  low titers. Every lot of this 2X One-Step RT-qPCR Master Mix has consistent performance across 6 magnitudes of SARS CoV-2 E-gene copies. The master mix is designed to use only a single assay protocol to test for either (or both) RNA and DNA and requires no passive dye.

High Sensitivity:  Performance of LaunchWorks produced master mix is comparable to ThermoFisher’s 4X TaqPath 1-Step Multiplex Master Mix. Across 6 magnitudes of the SARS CoV-2 E-gene, tested using FAM probe detection, the LaunchWorks produced master mix amplifies within 3 Ct of the Thermo Fisher master mix. As the copy number of E-gene present in the assay decreases, the LaunchWorks master mix amplification curve plateau reads stronger than the Thermo Fisher master mix.

Broad Instrument Compatibility:  The Launchworks 2X One-Step RT-qPCR Master Mix  performs across a wide range of real-time PCR systems. These systems include (but are not limited to) Applied Biosystems, BioRad CFX, Roche Lightcycler PCR platforms and Analytik Jena QTower 3.

High-Quality Enzymes:  The master mix is made up of 4 highly specific and active enzymes:  Reverse Transcriptase (RT), RNAse Inhibitor (RI), Taq DNA Polymerase (Taq), Uracil-DNA Glycosylase (UDG).

Manufactured in an ISO 13485:2016 certified facility under cGMP conditions.

Components:2X mix requiring no passive dye. Ten tubes in a kit, 100 reactions per tube
Storage Temp: -20°C or below
Shelf Life:12 months
Assay Name/Specification (minimum release criteria): 
 Launchworks 2X One-Step RT-qPCR Master Mix (No ROX) is functionally tested in One-Step RTqPCR with SARS CoV-2 E-gene RNA template, resulting in a standard curve across a dynamic range of 6 orders of magnitude
Individual Product Component Testing: Quality Control Tests including activity testing protocols are performed for each component included in the Launchworks 2X One-Step RT-qPCR Master Mix (No ROX) and meet the designated specifications of RNAse, DNAse and Protease Free

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2X One-Step RT-qPCR Master Mix, ten tubes each containing material for 100 reactions (1000 Reactions)
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2X One-Step RT-qPCR Master Mix, one tube containing material for 100 reactions, SAMPLE
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