X-GAL is histochemical substrate for β-galactosidase, allowing the detection of lac gene expression. To make a stock solution of X-GAL, dissolve the X-GAL in dimethylformamide to make a 20mg/ml solution.
Note:Use either a glass or polypropylene tube. Add 4ml of this solution per liter of culture media. The tube containing the solution should be wrapped in aluminum foil to prevent damage from light, and should be stored at -20°C.
Note: It is not necessary to sterilize X-GAL solutions by filtration.
Molecular Weight = 408.64 Purity = MIN 99.0%