The Vision Plate™ microplate range has been designed for high content screening (HCS) assays in drug development and related areas. It is also suitable for homogeneous assays employing fluorescence intensity, FRET and TR-FRET where measurements are bottom-read. This high quality optical base plate assures the necessary accuracy and consistency for automated high throughput systems, generating optimum signal to noise ratios. Using state-of-the-art manufacturing technology 4titude® has developed a product which offers several key advantages to the end user.
Optical Quality of the Polymer Film
The clear base component of our VisionPlate™ microplates demonstrates superior properties in terms of optical clarity (low absorbency and high transmission), low background fluorescence and consistency of material thickness.
The latest extrusion technology is used for manufacturing an ultra-clear base of 190 µm in thickness to provide optimum results with confocal microscopy and laser based detection systems.
Variation across the plate is minimised, so the time needed for complex screening applications can be reduced dramatically.
Reduction of Auofluorescence
4titude®’s VisionPlates™ are assembled using unique patented laser welding technology which reduces autofluorescence and does not inhibit cell growth. other competitors assemble clear base microplates by gluing a clear film to the frame or heat-welding the components together. Both techniques can cause problems with cell growth and the subsequent microscopic or fluorometric analysis.
Heat welding of the two plate components under high pressure results in autofluorescence at the well edges called “halo effect”. Gluing uses organic solvents within the adhesives, which can also cause autofluorescence. These solvents can also have cytotoxic effects, which may lead to inhibition of cell growth or even cell death. Additionally, incomplete glue lines or weld lines often result in well-to-well leakage.
4titude® offers coatings with Collagen 1 or Poly-D-lysine.
Collagen 1 is a protein of the extracellular matrix (ECM), an intercellular substance, which in vivo, influences adhesion, migration and proliferation among other processes. Collagen 1 is one of the most important ECM proteins for in-vitro cell cultures. Difficult to cultivate cells adhere to Collagen 1 and display positive growth. Collagen 1 can also influence differentiation and morphology.
Poly-D-lysine is a synthetic molecule that improves the adhesion of different cell types to polystyrene surfaces. It can improve cultivation efficiency of individual cell lines especially when serum-free or serum-reduced medium is used, or when experiments such as transfections are performed.
Azenta products are intended for sale/shipment within the US , Canada, and Latin America only. All other International customers should contact their local Azenta distributor to place orders for Azenta products.