p> ACTaq™ DNA Polymerase originates from Thermus Aquaticus with molecular weight of 94KDa. For use in DNA fragment extension amplification reactions with DNA having dA overhang on 3’ ends; at extension amplification speed of 1200 bases per minute and optimized between 65-75oC. Optimized for best performance when using dNTP concentration at 100-300mM, 1.5-3.0mM Mg2+ concentration, and pH at 8.1-9.2.
|Storage Condition:||--20°C. Stable for 12 months in constant freezer temperature|
|Unit Definition:||One unit of the amount of enzyme that incorporates 10nmols of dNTPs into|
acid-insoluble material in 30 minutes at 72°C
|Product Components:||ACTaq™ DNA Polymerase, PCR reaction buffer, 10X 25mM MgCl2|
|Storage Buffer:||10mM Tris (pH 8.2), 50mM KCL, 0.5% BSA, 0.5% NP-40, 50% glycerol|
|PCR Reaction Buffer:||10x PCR buffer with Mg2+. 15mM MgSO4, 100mM KCl, 80mM|
(NH4)2SO4, 100mM Tris-HCl, pH 9.0, 0.5%NP-50
|Quality Control:||ACTaq™ DNA Polymerase is highly purified. Free of contaminating|
endonucleases, exonucleases, and nicking activities. The purity of the
enzyme is evaluated by SDS-PAGE at >95% purity.