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ACTGene, Inc.

ACTaq™ Blue Hot-Strt DNAPolymerase

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ACTaq™ DNA Polymerase originates from Thermus Aquaticus with molecular weight of 94KDa. ACTaq™ Blue Hot-Start DNA Polymerase differs from ACTaq™ DNA Polymerase such that it contains antibody binding Taq which enables the activation of Taq only at 95oC. Additionally, it also comes with preloaded blue or red tracking and loading dye. The activation step eliminates the presence of non-specifics such as primer-dimer formation and mis-primed products, by ensuring the enzyme is inactive at temperature below 95oC. ACTaq™ Blue Hot-Start DNA Polymerase reduces the risk of contamination, making reaction preparation at ease when carried out in room temperature.

Hot-Start Activation Step:Pre-incubate 5 minutes at 95°C prior to thermal cycling
Storage Condition:-20°C. Stable for 12 months in constant freezer temperature
Concentration:5 Units/μL
Unit Definition:One unit of the amount of enzyme that incorporates 10nmols of dNTPs into
acid-insoluble material in 30 minutes at 72°C
Product Components:ACTaq™ Blue / Red Hot-Start DNA Polymerase (Antibody bound recombinant Taq DNA Polymerase); PCR reaction buffer; 10X 25mM MgCl2
Storage Buffer:10mM Tris (pH 8.2), 50mM KCL, 0.5% BSA, 0.5% NP-40, 50% glycerol
PCR Reaction Buffer:10x PCR buffer with Mg2+. 15mM MgSO4, 100mM KCl, 80mM
(NH4)2SO4, 100mM Tris-HCl, pH 9.0, 0.5%NP-50
Quality Control:ACTaq™ Blue / Red Hot-Start DNA Polymerase is highly purified. Free of contaminating endonucleases, exonucleases, and nicking activities. The purity of the enzyme is evaluated by SDS-PAGE at >95% purity.

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Product Detail
Thomas No.
C995K14
Mfr. No.
E3500B-250U
Also known as: DNP-E350B
Description
ACTaq™ Blue Hot-Start Taq DNA Polymerase, 250Units/Vial
list price/quantitytotal
$0.00
Thomas No.
C995K15
Mfr. No.
E3500B-1000U
Description
ACTaq™ Blue Hot-Start Taq DNA Polymerase, 1000Units/Vial
list price/quantitytotal
$0.00
$0.00 (0 Items)
Product is restricted and can only be purchased by customers with a web profile linked to a Thomas Business Account - Click here to login or create your web profile. If you do not have a Thomas Business Account, please Contact Us and someone will respond with details on how to apply for a business account with us.

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