PSB Broth Base is recommended for primary enrichment and enumeration of Yersinia enterocolitica from food. This medium is recommended by APHA for the primary enrichment of Yersinia species from food. Inoculate 25 grams of food sample to 2 of PSB Broth Base and incubate at 4deg C for 14-28 days. 1 mL of this primary enrichment is inoculated in 100 mL of PSTA Enrichment Broth Base (M940) and incubated at 28deg C for 48 hours. This secondary enrichment is then streaked on SS Agar (M108), Yersinia Selective Agar Base (M843).
Application: For Primary Enrichment and Enumeration of Yersinia Enterocolitica from Food