The Hamilton 79526 PRP-3 HPLC column has stainless-steel hardware and a PS-DVB polymer material type for reversed-phase absorption chromatography of organic compounds with large molecules that have a molecular weight (mw) greater than 2,000, including peptides, proteins, protein digests, protected and de-protected oligonucleotides, and nucleic acids. The stainless-steel hardware prevents particulates and highly adsorptive compounds from reaching the column. PS-DVB polymer columns resist chemical attack from organic solvents and aqueous buffers, which provides a longer life span as opposed to silica-based columns. PS-DVB supports are cross-linked to prevent shrinking and swelling, providing pressure stability up to 5,000 psi. The column has an organic compound classification with mobile phase limits of 1 to 13 pH, and 0 to 100% aqueous, organic modifier. The column has a 100-angstrom pore size, and filters 10-micron particles. It is 250mm long, and has a 10mm inside diameter (ID). HPLC columns are used with chromatography systems to perform high-performance liquid chromatography (HPLC) on a variety of organic and inorganic samples, including DNA, peptides, steroids, and metals.