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MilliporeSigma

Amicon Ultra-15 Centrifugal Filter Unit

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Provides both ultra-fast spin times and ultra-high recoveries

Amicon Ultra centrifugal filter units are the first devices to provide both ultra-fast spin times and ultra-high recoveries (typically >90%). The vertical membrane reduces concentration polarization for spin times as fast as 10–15 minutes. The heat-sealed membrane minimizes downstream extractables. The units are 100% integrity tested for reliable performance.

  • High recovery Ultracel regenerated cellulose membrane in a range of molecular weight cut-offs
  • High retentate recovery of >90%
  • Vertical membrane reduces concentration polarization for ultra-fast spin times (as fast as 10–15 minutes)
  • Heat-sealed membrane minimizes downstream extractables
  • 100% integrity tested for reliable performance
  • Convenient sample monitoring with translucent housing and volume gradations
  • Direct pipettor sample access eliminates processing step to recover concentrate
  • High concentration factors of 80–100X

Applications

  • Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms
  • Purification of macromolecular components found in tissue culture extracts or cell lysates and protein removal prior to HPLS
  • Concentration of dilute or pre-purified proteins from column eluents
  • Desalting, buffer exchange and protein dialysis
  • Size exclusion and protein fractionation
Volumes
Maximum Initial Sample Volume, mL*15
Typical Final Concentrate Volume, µL200
Centrifugal ForceSwinging bucket rotor: 4,000 x g; Fixed angle rotor (35°): 5,000 x g
Materials
MembraneUltracel regenerated cellulose
DeviceHousing: Styrene/butadiene
Tube: Polypropylene
Cap and liner: Polyethylene
Dimensions
Active Membrane Area, cm27.6
Diameter, mm29.7
Filtrate tube or vial (centrifuge tube)
Length, mm122
Filter unit
Diameter, mm29.6
Length, mm72.0
*Except for 35° fixed angle rotor: 12 mL

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Product Detail
Thomas No.
1211M23
Mfr. No.
UFC910096
Description
Amicon Ultra 15ml 100K 96pk
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