Salt Active Nuclease,

Storage: -20C

UNSPSC Code: 12352204

Biochem/physiol Actions: Salt active nuclease (SAN) is a general, unspecific endonuclease that cleaves double-stranded and single-stranded DNA, and RNA. SAN is active at above neutral pH.-á Unlike other nucleases, however, it has optimum activity at high concentrations of salt at high pH.-á These qualities make SAN ideal for removal of DNA from cell extracts and protein samples.-á The end-products upon complete degradation of DNA consist of a majority of 5 nucleotideoligos.-á The enzyme degrades DNA vs. RNA in a ratio of 10:1.SAN is highly active over the temperature range 10-40 C. The optimal NaCl-concentration for activity is 0.5 M.-á However, SAN is active in low salt buffers. -á1 M NaCl is not recommended for use of SAN.-á Mg2+ (>1 mM) is required for activity.-á The pH working range is above neutral pH, with an optimal range between pH 8.5-9.5Physical form: -áSolution in-á25 mM Tris-HCl (pH 7.5), 5 mM MgCl2, 0.5 M NaCl, 0.01% Triton, and 50% (v/v) glycerolUnit definition: -áOne unit (U) is defined as an increase in absorbance at 260 nm of 1 Absorbance Unit in 30 minutes at 37 -¦C, using 50 mug/ml calf thymus DNA in a buffer with 25 mM Tris-HCl (pH 8.5, 25 -¦C), 5 mM MgCl2, and 500 mM NaCl.