Synonyms: G3P Assay Kit
Storage: -20C
EC Number: 249-889-9
UNSPSC Code: 12352200
General description: Glycerol 3-phosphate (G3P) is an important intermediate of carbohydrate and lipid metabolic pathways. It is produced from glycerol by glycerol kinase or from dihydroxyacetone phosphate by glycerol 3-phosphate dehydrogenase. G3P may enter the G3P shuttle to generate NAD+, or may be converted to glyceraldehyde 3-phosphate and enter glycolysis or the lipid biosynthesis pathway.
Application: Glycerol-3-Phosphate Assay Kit has been used in the determination of glycerol-3-phosphate and phosphoenolpyruvate levels. It has also been used to quantify the concentration in aerial mycelium.
Principle: The Glycerol 3-Phosphate Colorimetric Assay Kit provides a simple assay for measuring G3P in various tissues and cells (ranging from 2-10 nmole/well). G3P is determined by measuring a colorimetric product with absorbance at 450 nm (A450) proportional to the amount of G3P present.
Suitability: Suitable for the detection of Glycerol-3-Phosphate in tissue and cell culture samples.
Components: G3P Assay Buffer; G3P Enzyme Mix; G3P Probe; G3P Standard
RIDADR: NONH for all modes of transport