Murashige and Skoog medium is a widely used plant tissue culture growth medium. M&S Basal Medium contains macronutrients that include high levels of nitrate and organic additives such as agar, sugars, vitamins and growth regulators. It has proven effective in the culture of tissue derived from moncotyledons and dicotyledons. This medium, originally formulated to support tobacco callus, has been used to support callus initiation and growth, the growth of cells in suspension culture and the regeneration of shoots and plantlets from explants. More recently, it has been used for micropropagation of ornamental, vegetable and fruit plants in research and commercial enterprises.
Murashige and Skoog basal salt medium includes the macro- and micronutrients as described by Murashige and Skoog, 1962.
Key Applications: Plant tissue culture growth
Application Areas: Plant Cell Growth
Product Type: Culture Media
Culture Media Type: Plant Media
Presentation: Off-white Powder
Format: Powder
Formulation: Components: Ammonium Nitrate: 1650.0 mg/L; Boric Acid: 6.2 mg/L; Calcium Chloride, Anhyd.: 332.2 mg/L; Cobalt Chloride 6H2O: 0.025 mg/L; Cupric Sulfate 5H2O: 0.025 mg/L; Na2- EDTA: 37.26 mg/L; Ferrous Sulfate 7H2O: 27.8 mg/L; Magnesium Sulfate: 180.7 mg/L; Manganese Sulfate: 16.9 mg/L; Molybdic Acid, Sodium Salt, 2H2O: 0.25 mg/L; Potassium Iodide: 0.83 mg/L; Potassium Nitrate: 1900.0 mg/L; Potassium Phosphate, Monobasic: 170.0 mg/L; Zinc Sulfate, 7H2O: 8.60 mg/L; Grams of powder to prepare 1 liter: 4.3 mg/L
pH: 3.5 - 4.5 (4.33 g/L)
NOTES: Biological: Supports or Facilitate Plant Growth
Solubility: Soluble in water (1×, Clear, Colorless to Clear with Slight Yellow Tint). Soluble in water (4.331 g/L).
Storage & Handling: Store at +4°C, desiccate.