RNase A: Product Description A major application for Ribonuclease A (RNase A) is the removal of RNA from preparations of plasmid DNA. In this application, the presence of DNase activity as an impurity is a concern. The boiling-water bath method used to eliminate contaminating DNase activity has proven unreliable. For this reason, Bio Basic Inc. developed a proprietary chromatographic preparation method for elimination of DNase activity.
RNase A is an endoribonuclease that attacks at the 3¢ phosphate of a pyrimidine nucleotide. The sequence of pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG. The highest activity is exhibited with single stranded RNA. RNase A is a single chain polypeptide containing 4 disulfide bridges. In contrast to RNase B, it is not a glycoprotein. RNase A can be inhibited by alkylation of His12 or His119 , which are present in the active site of the enzyme. Activators of RNase.
Molecular mass: 13.7 kDa (amino acid sequence)
Extinction coefficient: E1% = 7.1 (280 nm) Isoelectric point: pI = 9.6
Optimal temperature: 60 °C (activity range of 15–70 °C)
Optimal pH: 7.6 (activity range of 6–10)
Inhibitors: ribonuclease inhibitor
The chromatographically purified product is supplied as an essentially salt-free lyophilized powder.
Activity (Kunitz): ≥60 units/mg protein