Yeast Nitrogen Base (YNB) w/ Ammonium Sulphate is used for the growth of all strains of Saccharomyces cerevisiae. This medium has been formulated after following the procedures of Wickerham and Burkholder (1, 2, 3). This is a well defined medium consisting of salts, vitamins and ammonium sulphate (nitrogen source). Addition of a carbon source is required for the growth of Sacharomyces cerevisiae. YNB w/ Ammonium Sulphate is formulated for investigation of yeast for their differential abilities in carbon assimilation in the presence of a carbon source like glucose, galactose or raffinose.
References:
1. Wickerham L. J., 1951, U.S. Dept. Agric. Tech. Bull. No. 1029
2. Wickerham L. J., 1946, J. Bacteriol., 52:293
3. Burkholder, P. R. 1943. Vitamin deficiencies in yeasts. Amer. J. Bot. 30:206-211
Appearance of Powder:
Cream to yellow coloured, homogeneous, free flowing powder.
Color and Clarity of prepared medium:
Light yellow coloured, clear solution without any precipitate.
Cultural Response:
Cultural characteristics observed after an incubation at 25-30°C for 18 - 48 hours.
| Organisms (ATCC) | Growth (plain) | Growth (with Dextrose) |
| Saccharomyces cerevisiae | Poor-good | good-luxuriant |
Storage and Shelf-life:
Store below 30°C and the prepared medium at 2 - 8°C. Use before expiry date on the label.
SD Growth Medium w/o URA
SD Growth Medium w/o URA is a synthetic defined media for the selective growth of Saccharomyces cerevisiae.
| Composition**: |
| Ingredients | Milligrams/Litre |
| Potassium phosphate, monobasic | 1000.00 |
| Magnesium sulphate | 500.00 |
| Sodium chloride | 100.00 |
| Calcium chloride | 100.00 |
| D-Biotin | 0.002 |
| Calcium pantothenate | 0.40 |
| Folic acid | 0.002 |
| Inositol | 2.00 |
| Niacin | 0.40 |
| PABA | 0.20 |
| Pyridoxin, HCl | 0.40 |
| Riboflavin | 0.20 |
| Thiamine HCl | 0.40 |
| Boric acid | 0.50 |
| Copper sulphate | 0.04 |
| Potassium iodide | 0.10 |
| Ferric chloride | 0.20 |
| Manganese sulphate | 0.40 |
| Sodium molybdate | 0.20 |
| Zinc sulphate | 0.40 |
| Ammonium sulphate | 5000.00 |
| ** Formula adjusted, standardized to suit performance parameters |