Tryptose Broth w/ Thiamine HCl is used for the isolation, cultivation and differentiation of fastidious microorganisms in an infusion free medium. Tryptose containing media, rather than the conventionally used meat infusion media have been used for the enumeration and isolation of Brucella species. These media can be used as general purpose media for cultivation of wide variety of organisms. Supplementation of the medium with defibrinated blood (sheep, horse) to prepare blood agar aids in the isolation of fastidious organisms.
- Appearance: Cream to yellow homogeneous free flowing powder
- Color and Clarity of Prepared medium: Basal Medium : Yellow colored clear to slightly opalescent Solution. After addition of 5% v/v sterile defibrinated blood: cherry red colored opaque solution forms in tubes
- Reaction of 2.6% w/v aqueous solution at 25°C. pH : 7.2±0.2
- M997: Cultural characteristics observed after an incubation at 35-37°C for 48-72 hours with added 5% v/v sterile defibrinated blood in presence of 10% Carbon dioxide (CO2)