LB Broth (Miller formulation) with Kanamycin is used for the growth and maintenance of E. coli containing recombinant plasmids. LB Miller media is nutritionally rich, which allows for rapid growth.
Kanamycin is used to select for mutated or transformed cells that are kanamycin resistant. In research labs, there are several commonly used kanamycin resistance genes (e.g., aph, ntpII, and aaC-aphD). Typically, low-copy-number plasmids require less kanamycin than high-copy-number plasmids. Note: 50 µg/mL kanamycin is the most commonly used concentration.
Contains 1.0% (10 g/L) tryptone, 0.5% (5 g/L) yeast extract, 1.0% (10 g/L) sodium chloride, and kanamycin (amount varies by part number).