Plate Count Agar, special is used for the enumeration of viable bacteria in raw milk, milk and other diary products (1).
Casein enzymic hydrolysate provides amino acids and other complex nitrogenous substances. Yeast extract supplies Vitamin B-complex. Generally pour plate technique is followed. The samples are diluted and appropriate dilutions are placed in Petri plates. Sterile molten agar is added to these plates and plates are rotated gently to ensure uniform mixing of the sample with agar.
Storage and Shelf-life:
Store below 30°C in tightly closed container and the prepared medium at 2-8°C. Use before expiry date on the label.
Reference:
1. Atlas R.M., 1993, Handbook of microbiological Media, CRC Press, Inc.