High salt formula. Molecular Biology Grade. For maintenance and propagation of E. Coli.
RPI Media is now available in convenient pre-measured and pre-mixed capsules. Prebuffered media eliminates messy weighing and pH adjustments.
Simply dissolve 25 capsules per 1 Liter of distilled water. Water must be 37°C or warmer to completely dissolve gelatin capsule. Agitate. Sterilize by autoclaving for 15 minutes before use. Buffer is set for 7.0 ± 0.2 at 25°C using a 10mM solution of Tris/Tris HCl. pH will vary depending on temperature.
- Tryptone: 10 g/L
- Sodium Chloride: 10 g/L
- Yeast Extract: 5 g/L
- Tris/Tris HCl: 1.5 g/L