It can be used for cultivation of fastidious microorganisms in Columbia Broth Base HiVeg (MV145),
Dextrose HiVeg Agar (MV044), Casman HiVeg Agar (MV201) etc. Large scale production of antibiotics, enzymes and
other products of microbiological origin.
Principle And Interpretation
HiVeg Peptone No.5 is a mixture of enzymic digests of vegetable proteins. It has high nutritive values to meet the growth
requirements of wide variety of microorganisms. It can successfully replace Biopeptone (RM021).
Quality Control
Appearance
Light yellow to yellow, may have a slight green tinge Homogenous Free flowing having Characteristic odour of protein, derived
from vegetable source.
Solubility
Freely soluble in distilled/purified water,insoluble in alcohol and ether.
Clarity
1% w/v aqueous solution is clear without any haziness after autoclaving at 15 lbs pressure (121ºC ) for 15 minutes.
Reaction
Reaction of 2%w/v aqueous solution at 25ºC.
pH
5.50- 7.50
Microbial Load:
Total aerobic microbial count (cfu/gm)
By plate method when incubated at 30-35°C for not less than 3 days.
Bacterial Count : <= 2000 CFU/gram
Total Yeast and mould count (cfu/gm)
By plate method when incubated at 20-25°C for not less than 5 days.
Yeast & mould Count : <= 100 CFU/gram
Test for Pathogens
1. E.coli-Negative in 10 gms of sample2. Salmonella species-Negative in 10 gms of sample3. Pseudomonas aeruginosa-
Negative in 10 gms of sample4. Staphylococcus aureus- Negative in 10 gms of sample5. C.albicans- Negative in 10 gms of
sample6. Clostridia- Negative in 10 gms of sample
Indole test
Tryptophan content: Passes
Cultural response
Cultural response observed after incubation at 35 - 37°C for 18-48 hours by preparing Columbia Broth Base, HiVeg
(MV145),using HiVeg Peptone No.5 as an ingredient.
Storage and Shelf Life
Store below 30°C. Use before expiry date on the label.