Principle And Interpretation
HiVeg Hydrolysate No. 2 is an enzymic digest of vegetable proteins and contains highly nutritive ingredients required
for cultivation of nutritionally demanding microorganisms. Growth response of this hydrolysate is comparable with Liver
Hydrolysate (RM023). It can also be recommended for large scale cultivation of these bacteria for the purpose of vaccine
Light yellow to yellow may have slight green tinge ,Homogenous Free flowing powder, having Characteristic odour but not putrescent.
Freely soluble in distilled/purified water,insoluble in alcohol and ether.
1% w/v aqueous solution is clear without any haziness after autoclaving at 15 lbs pressure (121ºC ) for 15 minutes.
Reaction of 2%w/v aqueous solution at 25ºC.
Total aerobic microbial count (cfu/gm)
By plate method when incubated at 30-35°C for not less than 3 days.
Bacterial Count : <= 2000 CFU/gram
Total Yeast and mould count (cfu/gm)
By plate method when incubated at 20-25°C for not less than 5 days.
Yeast & mould Count : <= 100 CFU/gram
Test for Pathogens
1. E.coli-Negative in 10 gms of sample2. Salmonella species-Negative in 10 gms of sample3. Pseudomonas aeruginosa-
Negative in 10 gms of sample4. Staphylococcus aureus- Negative in 10 gms of sample5. C.albicans- Negative in 10 gms of
sample6. Clostridia- Negative in 10 gms of sample
Tryptophan content: Passes
Cultural response observed after an incubation at 35-37°C for 18-24 hours by preparing Liver Infusion HiVeg Agar
(MV374) using HiVeg Hydrolysate No. 2 as an ingredient.
Storage and Shelf Life
Store below 30°C. Use before expiry date on the label.