It can be used in following media: Tryptose Media : for cultivation of fastidious microorganisms and for preparing Blood
Agars, HiVeg (MV073/MV834). HiVeg hydrolysate No. 1 aids in maintaining blood cells in excellent state, thus haemolytic
reactions can be easily demonstrated.Vaccine Preparation Media : for rapid and luxuriant growth as desired for large scale
manufacturing of vaccines and toxins..
Principle And Interpretation
HiVeg Hydrolysate No. 1 is specially prepared enzymic hydrolysate of vegetable proteins that can successfully replace Tryptose (RM030) (Milk Protein).
Light yellow to yellow,may have slightly green tinge Homogenous Free flowing powder,having Characteristic but not putrescent odour.
Freely soluble in distilled/purified water,insoluble in alcohol and ether.
1% w/v aqueous solution is clear without any haziness after autoclaving at 15 lbs pressure (121ºC ) for 15 minutes.
Reaction of 2%w/v aqueous solution at 25ºC.
Total aerobic microbial count (cfu/gm)
By plate method when incubated at 30-35°C for not less than 3 days.
Bacterial Count : <= 2000 CFU/gram
Total Yeast and mould count (cfu/gm)
By plate method when incubated at 20-25°C for not less than 5 days.
Yeast & mould Count : <= 100 CFU/gram
Test for Pathogens
1. E.coli-Negative in 10 gms of sample2. Salmonella species-Negative in 10 gms of sample3. Pseudomonas aeruginosa-
Negative in 10 gms of sample4. Staphylococcus aureus- Negative in 10 gms of sample5. C.albicans- Negative in 10 gms of
sample6. Clostridia- Negative in 10 gms of sample
Tryptophan content: Passes
Cultural response observed after an incubation at 35-37ºC for 18-24 hours by preparing Lauryl Tryptose HiVeg Broth
(MV080) using HiVeg Hydrolysate No. 1 as an ingredient.
Storage and Shelf Life
Store below 30°C. Use before expiry date on the label.