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EMB Agar (Levine)

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Levine EMB Agar was developed by Levine (1,2) and is used for the differentiation of Escherichia coli and Enterobacter aerogenes and also for the rapid identification of Candida albicans. This medium is recommended for the detection, enumeration and differentiation of members of the coliform group by American Public Health Association (3, 4, 5). It is also recommended by BIS for detection and estimation of coliform bacteria in food stuff (6) and Escherichia coli from food and water (7). Eosin-Y and methylene blue make the medium slightly selective and inhibit certain gram-positive bacteria. These dyes differentiate between lactose fermenters and nonfermenters. Some gram-positive bacteria such as faecal Streptococci, yeasts grow on this medium and form pinpoint colonies. Weld (8, 9) proposed the use of Levine EMB Agar, with added Chlortetracycline hydrochloride, for the rapid identification of Candida albicans in clinical specimens. A positive identification of Candida albicans can be made after 24 - 48 hours incubation at 35 - 37°C in 10% carbon dioxide atmosphere, from specimens such as faeces, oral and vaginal secretions and nail or skin scraping etc. However, the typical appearance is variable.

Storage and Shelf-life:
Store below 30°C in tightly closed container and prepared medium at 2-8°C and away from light. Use before expiry date on the label.

Quality Control
Appearance Light pink to purple colored homogeneous free flowing powder
Gelling Firm, comparable with 1.5% Agar gel
Color and Clarity of prepared medium Reddish purple coloured slightly opalescent gel with greenish cast and finely dispersed precipitate, forms in Petri plates.
Reaction Reaction of 3.75% w/v aqueous solution at 25°C. pH : 7.1±0.1
Cultural Response M022S: Cultural characteristics observed after an incubation at 35 - 37°C for 24-48 hours.
Ingredients Gms/Litre
Peptic digest of animal tissue 10.000
Dipotassium phosphate 2.000
Lactose 10.000
Eosin - Y 0.400
Methylene blue 0.065
Agar 15.000
Final pH ( at 25°C) 7.1±0.1
**Formula adjusted, standardized to suit performance parameters

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Thomas No.
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EMB Agar (Levine), 500 g

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Thomas No.
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EMB Agar (Levine), 2.5 kg

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EMB Agar (Levine), 5 kg

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