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HiMedia

Casein Enzyme Hydrolysate, Type-II

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It is used in Antibiotic Assay Media owing to its outstanding clarity in solution and is also used in media for commercial antibiotic production media.

Casein Enzyme Hydrolysate, Type II is carefully manufactured under controlled conditions of enzyme hydrolysis using high quality lactose free casein and is equivalent to Casitone and is specially used in Antibiotic Assay Media. It is exceptionally light in color as compared to other Casein Hydrolysates and forms a very light colored solution which is extremely clear,rendering it ideal in culture media wherein microbial growth is determined by optical means. Also it is a very rich source of Amino Nitrogen.

Appearance
Off white to light yellow Homogenous Free flowing powder, having Characteristic odour but not putrescent.

Solubility
Freely soluble in distilled/purified water, insoluble in alcohol and ether.

Clarity
1% w/v aqueous solution is clear without any haziness after autoclaving at 15 lbs pressure (121ºC) for 15 minutes.

Reaction
Reaction of 2% w/v aqueous solution at 25ºC.

Storage and Shelf Life
Store below 30°C. Use before expiry date on the label.

pH6.20- 7.20
Total aerobic microbial count (cfu/gm)By plate method when incubated at 30-35°C for not less than 3 days.
Bacterial Count : <= 2000 CFU/gram
Total Yeast and mold count (cfu/gm)By plate method when incubated at 20-25°C for not less than 5 days.
Yeast & mold Count : <= 100 CFU/gram
Test for Pathogens1. E.coli-Negative in 10 gms of sample 2. Salmonella species-Negative in 10 gms of sample 3. Pseudomonas aeruginosa-Negative in 10 gms of sample 4. Staphylococcus aureus- Negative in 10 gms of sample 5. C.albicans- Negative in 10 gms of
sample 6. Clostridia- Negative in 10 gms of sample
Indole TestTryptophan content: Passes
Cultural responseCultural response observed after an incubation at 35-37ºC for 18-48 hours by preparing Soyabean Casein Digest Medium (M011) using Casein Enzyme Hydrolysate Type II as an ingredient.

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Thomas No.
C985J61
Mfr. No.
RM028-500G
Description
Casein Enzyme Hydrolysate, Type-II, 500g
list price/quantitytotal
$0.00
Thomas No.
C985J62
Mfr. No.
RM028-2.5KG
Description
Casein Enzyme Hydrolysate, Type-II, 2.5kg
list price/quantitytotal
$0.00
Thomas No.
C985J63
Mfr. No.
RM028-5KG
Description
Casein Enzyme Hydrolysate, Type-II, 5kg
list price/quantitytotal
$0.00
Thomas No.
C985J64
Mfr. No.
RM028-10KG
Description
Casein Enzyme Hydrolysate, Type-II, 10kg
list price/quantitytotal
$0.00
Thomas No.
C985J65
Mfr. No.
RM028-25KG
Description
Casein Enzyme Hydrolysate, Type-II, 25kg
list price/quantitytotal
$0.00
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