Actidione Agar was formulated by Green and Gray (1) which may be used for microbiological investigation during brewing and baking. Actidione® (Cycloheximide) at a concentration of 0.001% permits the growth of bacteria and inhibits the growth of most yeasts and moulds except dermatophytes. This medium may be used for the estimation of bacterial contamination of pitching yeast. Addition of penicillin or streptomycin may be used for selective isolation of dermatophytes.
Casein enzymic hydrolysate acts as source of nitrogen while yeast extract is a rich reservoir of vitamins. Dextrose in high amount along with mineral salts at acidic pH favors sugar fermentation.
Storage and Shelf-life:
Store below 30°C in tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on label.
Reference:
1.Green, S.R. and Gray, P.P., 1950, Wallerstein Lab. Communication, 13:357.