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BioSpectrometer® fluorescence

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BioSpectrometer® fluorescence

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The Eppendorf BioSpectrometers are spectrophotometers that combine easy handling with highly reproducible analysis. A variety of cuvettes expands the options even further from microvolume to macrovolume measurements.

The Eppendorf BioSpectrometer fluorescence is an expansion of the Eppendorf BioSpectrometer basic and features an integrated fluorescence module. Measurements can be flexibly executed in the UV/Vis or fluorescence range. The Eppendorf BioSpectrometer fluorescence can be used to increase the measuring range for biomolecules (nucleic acids and proteins) by a factor of 1,000, compared to UV measurements. What's more, fluorescence dyes allow a more accurate and reliable quantification due to the fact that these dyes bind specifically to the target biomolecule. UV/Vis spectroscopy and fluorescence are complementary methods. The Eppendorf BioSpectrometer fluorescence is the first spectrophotometer on the market which combines both measuring methods in one small, compact device. In combination with the microvolume cuvette, Eppendorf µCuvette® G1.0, it can measure dsDNA from 1 pg/µL to 1,500 ng/µL.


  • All Eppendorf BioSpectrometer basic options
  • Preprogrammed and freely programmable fluorescence methods
  • Fluorescence measurement concentration of dsDNA: 1 pg/µL – 1,000 pg/µL
  • Fluorescence measurement concentration of BSA: 0.01 ng/µL – 10 ng/µL
  • Fluorescence excitation wavelength 470 nm, emission wavelengths 520 nm and 560 nm
  • Fluorescence intensity across a range of 0.5 nM to 2,000 nM fluorescein


  • dsDNA quantification down to 1 pg/µL with common kits, e.g., Pico Green®
  • ssDNA quantification down to 10 pg/µL
  • RNA quantification down to 20 pg/µL
  • Oligo quantification down to 10 pg/µL
  • Protein (BSA) quantification down to 0.01 ng/µL
  • Quantification of GFP-binding proteins
  • Growth measurement of GFP-binding bacteria

The Eppendorf BioSpectrometer Fluorescence Reference Filter Set: is used to verify photometric accuracy, wavelength systematic error, and fluorimetric precision (random error) and linearity on the Eppendorf BioSpectrometer fluorescence.

 BioSpectrometer® kinetic
Absorption measuring range0A–3.0A (260nm)
BSA concentration range0.01 ng/µL – 45.45 µg/µL
BSA concentration range (UV 280 nm) µCuvette G1.075 ng/µL – 45.45 µg/µL
±0.03 µg/µL at A = 0
±0.076 µg/µL at A = 1 (0.5 %)
BSA concentration range (UV 280 nm) UVette 10 mm7.5 ng/µL – 4.5 µg/µL
±0.003 µg/µL at A = 0
±0.007 µg/µL at A = 1 (0.5 %)
BSA concentration range (UV 280 nm) UVette 2 mm37.5 ng/µL – 22.5 µg/µL
±0.015 µg/µL at A = 0
±0.038 µg/µL at A = 1 (0.5 %)
Beam receiver absorptionCMOS photodiode array
Beam receiver fluorescencePhotodiodes
Cuvette shaft12.5x12.5mm
Emission wavelength I fluorescence520nm, bandwidth: 15nm
Emission wavelength II fluorescence560nm, bandwidth: 40nm
Excitation wavelength fluorescence470nm, bandwidth: 25nm
Fluorescence measuring range0.5nM–2,000nM fluorescein (emission wavelength 520nm)
Light path height8.5mm
Light source absorptionXenon flash lamp
Light source fluorescenceLED
Measuring principle absorptionSingle-beam absorption spectrophotometer with reference beam
Measuring principle fluorescenceConfocal filter fluorometer with reference beam
Method memory>100 method programs
Mode absorptionSpectrophotometer
Mode fluorescenceFluorometer
Operator guidance languageSpanish, Italian, French, English, German
Power consumptionApprox. 15W during operating step
Approx. 5W with dimmed display
Random error absorption≤0.002 at A=0
≤0.005 (0.5%) at A=1
Random error fluorescence±2 % at 1nM fluorescein
Random error wavelength±1 nm
Random temperature error
Smallest step size1 nm
Spectral bandwidth≤4 nm
Systematic error absorption±1 % (A = 1)
Systematic error wavelength≤0.5 nm
Systematic temperature error
Wavelength range200nm–830nm
Wavelength range absorptionScan (nm): 200 – 830 at 1 nm increments
dsDNA concentration range1.0 pg/µL – 1,500 ng/µL
dsDNA concentration range (UV 260 nm) UVette 10 mm2.5 ng/µL – 150 ng/µL
±0.1 ng/µL at A = 0
±0.25 ng/µL at A = 1 (0.5 %)
dsDNA concentration range (UV 260 nm) UVette 2 mm12.5 ng/µL – 750 ng/µL
±0.5 ng/µL at A = 0
±1.25 ng/µL at A = 1 (0.5 %)
dsDNA concentration range (UV 260 nm) µCuvette G1.025 ng/µL – 1,500 ng/µL
±1 ng/µL at A = 0
±2.5 ng/µL at A = 1 (0.5 %)
Interfaces› USB master: for USB stick and thermal printer DPU-S445
› USB slave for connecting to a PC
› Serial interface RS-232: for thermal printer DPU-414
› Ethernet interface RJ45: For connecting to a network printer
Power supply100–120V, 50–60 Hz
Dimensions (W?x?D?x?H)295 x 400 x 150mm / 11.6 x 15.7 x 6"
Weight w/o accessories5.4 kg
Interval between two measuring points
Measuring time range
Adjustable temperature range

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BioSpectrometer® fluorescence Reference Filter Set
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