The Eppendorf BioSpectrometers are spectrophotometers that combine easy handling with highly reproducible analysis. A variety of cuvettes expands the options even further from microvolume to macrovolume measurements.
The Eppendorf BioSpectrometer fluorescence is an expansion of the Eppendorf BioSpectrometer basic and features an integrated fluorescence module. Measurements can be flexibly executed in the UV/Vis or fluorescence range. The Eppendorf BioSpectrometer fluorescence can be used to increase the measuring range for biomolecules (nucleic acids and proteins) by a factor of 1,000, compared to UV measurements. What's more, fluorescence dyes allow a more accurate and reliable quantification due to the fact that these dyes bind specifically to the target biomolecule. UV/Vis spectroscopy and fluorescence are complementary methods. The Eppendorf BioSpectrometer fluorescence is the first spectrophotometer on the market which combines both measuring methods in one small, compact device. In combination with the microvolume cuvette, Eppendorf µCuvette® G1.0, it can measure dsDNA from 1 pg/µL to 1,500 ng/µL.
Features
- All Eppendorf BioSpectrometer basic options
- Preprogrammed and freely programmable fluorescence methods
- Fluorescence measurement concentration of dsDNA: 1 pg/µL – 1,000 pg/µL
- Fluorescence measurement concentration of BSA: 0.01 ng/µL – 10 ng/µL
- Fluorescence excitation wavelength 470 nm, emission wavelengths 520 nm and 560 nm
- Fluorescence intensity across a range of 0.5 nM to 2,000 nM fluorescein
Applications
- dsDNA quantification down to 1 pg/µL with common kits, e.g., Pico Green®
- ssDNA quantification down to 10 pg/µL
- RNA quantification down to 20 pg/µL
- Oligo quantification down to 10 pg/µL
- Protein (BSA) quantification down to 0.01 ng/µL
- Quantification of GFP-binding proteins
- Growth measurement of GFP-binding bacteria
The Eppendorf BioSpectrometer Fluorescence Reference Filter Set: is used to verify photometric accuracy, wavelength systematic error, and fluorimetric precision (random error) and linearity on the Eppendorf BioSpectrometer fluorescence.