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BioSpectrometer® basic

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BioSpectrometer® basic

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The Eppendorf BioSpectrometer® basic makes your work easier with the following features: simplest handling, guided software procedures, operation directly on the device, preprogrammed methods, automatic evaluation of measurement data and clear presentation of results.

The Eppendorf BioSpectrometer basic is a small, very compact spectrophotometer that combines easy handling with highly reproducible analysis. It can measure and record UV/Vis spectral ranges as well as measure individual wavelengths from 200 nm to 830 nm. Its freely selectable wavelengths provide maximum flexibility for all current and future applications. In addition to preprogrammed methods for standard measurements, users can create and store their own methods. Optimized menu navigation guides through the individual methods in a step-by-step process. All required entries are visible, no essential data is omitted. A help box explains the operational sequences and is available in 5 different languages.


  • Absorbance measurement for one or more wavelengths, recording of wavelength scans from 200 nm to 830 nm (increment: 1 nm)
  • Automatic evaluation and storage of measurement data with clear presentation of results
  • Spectral graph to display sample purity with automatic ratio calculation
  • Preprogrammed applications for quick start
  • Freely programmable applications with evaluation via factor, standard or standard series and freely programmable parameters
  • Two-wavelength method with substraction or division evaluation
  • Integrated application and results memory
  • Data transfer via USB interface, Ethernet or via e-mail and direct printout of results
  • Guided software process to minimize errors
  • Integrated self-test and calibration history


  • Nucleic acid quantification
  • Direct protein quantification (UV 280 nm)
  • Microvolume measurement via µCuvette G1.0 for highly concentrated samples
  • Bacteria growth measurement (OD 600)
  • Colorimetric assays for protein quantification, e.g., BCA, Bradford, Lowry
  • Evaluation of FOI for dye-labeled biomolecules (nucleic acid or proteins)
  • Free selectable wavelengths, e.g., 340 nm: assays using NADPH or NAPH, 405 nm: assays using para-nitrophenol, 420 nm: assays using ortho-nitrophenol, 490 nm: colorimetric fructose determination, 490 nm: cytotoxicity assay
 BioSpectrometer® kinetic
Absorption measuring range0 A – 3.0 A (260 nm)
BSA concentration range7.5 ng/µL – 45.45 µg/µL
BSA concentration range (UV 280 nm) µCuvette G1.075 ng/µL – 45.45 µg/µL
±0.03 µg/µL at A = 0
±0.076 µg/µL at A = 1 (0.5 %)
BSA concentration range (UV 280 nm) UVette 10 mm7.5 ng/µL – 4.5 µg/µL
±0.003 µg/µL at A = 0
±0.007 µg/µL at A = 1 (0.5 %)
BSA concentration range (UV 280 nm) UVette 2 mm37.5 ng/µL – 22.5 µg/µL
±0.015 µg/µL at A = 0
±0.038 µg/µL at A = 1 (0.5 %)
Beam receiver absorptionCMOS photodiode array
Cuvette shaft12.5 mm x 12.5mm
Emission wavelength I fluorescence
Emission wavelength II fluorescence
Excitation wavelength fluorescence
Fluorescence measuring range
Light path height8.5mm
Light source absorptionXenon flash lamp
Light source fluorescence
Measuring principle absorptionSingle-beam absorption spectrophotometer with reference beam
Measuring principle fluorescence
Method memory>100 method programs
Operator guidance languageSpanish, Italian, French, English, German
Power consumptionApprox. 15W during operating step
Approx. 5W with dimmed display
Random error absorption≤0.002 at A=0
≤0.005 (0.5%) at A=1
Random error fluorescence
Random error wavelength±1 nm
Random temperature error
Smallest step size1 nm
Spectral bandwidth≤4 nm
Systematic error absorption±1 % (A = 1)
Systematic error wavelength≤0.5 nm
Systematic temperature error
Wavelength range200nm–830nm
Wavelength range absorptionScan (nm): 200 – 830 at 1 nm increments
dsDNA concentration range2.5 ng/µL – 1,500 ng/µL
dsDNA concentration range (UV 260 nm) UVette 10 mm2.5 ng/µL – 150 ng/µL
±0.1 ng/µL at A = 0
±0.25 ng/µL at A = 1 (0.5 %)
dsDNA concentration range (UV 260 nm) UVette 2 mm12.5 ng/µL – 750 ng/µL
±0.5 ng/µL at A = 0
±1.25 ng/µL at A = 1 (0.5 %)
dsDNA concentration range (UV 260 nm) µCuvette G1.025 ng/µL – 1,500 ng/µL
±1 ng/µL at A = 0
±2.5 ng/µL at A = 1 (0.5 %)
Interfaces› USB master: for USB stick and thermal printer DPU-S445
› USB slave for connecting to a PC
› Serial interface RS-232: for thermal printer DPU-414
› Ethernet interface RJ45: For connecting to a network printer
Power supply100–120V, 50–60 Hz
Dimensions (W?x?D?x?H)295x 400x 150mm/ 11.6x 15.7x 6"
Weight w/o accessories5.4 kg
Interval between two measuring points
Measuring time range
Adjustable temperature range

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