3D Cell Culture

3D in a fast and easy 2D workflow


The core technology of Greiner Bio-One’s Magnetic 3D Cell Culture is the magnetization of cells with biocompatible NanoShuttle™-PL. The reproducible formation of one spheroid per well in an F-bottom plate with Cell-Repellent surface is forced by magnets either by levitation or bioprinting, to form structurally and biologically representative 3D models in-vitro

Advantages of the magnetic 3D cell culture (m3D) technology are:

  • 3D in a 2D workflow
  • Reproducible spheroid formation
  • Scalable – 6 Well to 1536 Well
  • Performed on a flat surface optimal for high-resolution microscopy and HTS
  • Rapid 3D culture formation within 24 hours for most cell types
  • No specialized equipment or media
  • Easy media changes and co-culture of different cell types
  • Compatible with fluorescence microscopy, Western blotting, qRT-PCR, Flow Cytometry, viability assays, chemiluminescence, etc.
  • Ready for automation
  • Biocompatible nanoparticles to magnetize cells


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