IsoPure™ 96, Automated Purification System

The IsoPure™ 96 from Accuris Instruments is a high throughput, automated system that improves the efficiency and reproducibility of magnetic bead-based purification protocols. Capable of processing up to 96 samples at one time, the instrument is easy to set up and program. Mixing, magnetic bead transfer, washing, and elution steps are performed automatically. The purification process can be completed within 10 to 60 minutes depending on the type of reagent kit used and complexity of the program.

Programming
The IsoPure 96 can be programmed by using the color touch screen or the included mouse. After a protocol has been created, the instrument will calculate and display the estimated time for each step and the total time.

Typical step settings include:

Tip Comb Loading and Unloading - Automate tip comb loading and unloading by designating the respective plate positions in the script.

Mixing - Parameters for mixing time, speed, and amplitude can be programmed for each step of the protocol.

Temperature Control - Heating of the sample mixture during the lysis and elution steps significantly improves the purity and yield of the target molecule. The IsoPure 96 is equipped with 2 heating blocks on the plate carousel that can be precisely set to temperatures from ambient + 5°C to 120°C. IsoPure deep well plates fit into the heating blocks for efficient heat transfer. A cooling fan inside the processing chamber maintains the ambient operating temperature to ensure consistent results.

Bead Collection and Release - The complete collection of magnetic beads from sample mixtures is crucial for maximizing the yield of purified analytes. The IsoPure software offers full control of magnetic parameters to ensure cleaner extractions. General settings allow control of magnet speed, position, repeat collection cycles, etc, to guarantee consistent yields.

Protocol Optimization - Accuris Instruments has partnered with leading magnetic bead kit manufacturers to develop and optimize protocols for specific applications. To request protocols for specific kits, or for assistance in optimization, please contact the IsoPure Technical Support Team at info@accuris-usa.com.

IsoPure™ Consumables - Accuris offers high-quality deep well plates and magnetic tip combs designed to fit the IsoPure 96. Produced using virgin polypropylene and packaged in a sterile facility, these consumables are guaranteed to be contaminant-free. The IsoPure 96 also accepts industry-standard 96 deep well plates and tip combs designed for high throughput purification systems.