Silica-based HPLC phases for anion exchange chromatography of nucleic acids
Features
- Base material silica, particle size 7 µm
- DEAE anion exchanger
NUCLEOGEN® 60-7 DEAE:
- Pore size 60 Å
- Capacity 200 A260/mL (~ 300 A260 for a 125 x 4 mm ID column, 1875 A260 for a 125 x 10 mm ID column)
- For separation of oligonucleotides up to chain lengths of 40 bases with recoveries > 95 %
- Preparative separations possible when using higher flow rates and longer gradient times
NUCLEOGEN® 500-7 DEAE:
- Pore size 500 Å
- Capacity 730 A260 for a 125 x 6 mm ID column, 1940 A260 for a 125 x 10 mm ID column
- For the separation of tRNA, 5S RNA, viroids and messenger RNA in the intermediate molecular weight range (25,000–1,000,000 daltons) with recoveries > 95 %
NUCLEOGEN® 4000-7 DEAE:
- Pore size 4000 Å
- Capacity 120 A260 for a 125 x 6 mm ID column, 350 A260 for a 125 x 10 mm ID column
- For the separation of plasmids, DNA restriction fragments, ribosomal RNA, messenger RNA and viral RNA, i.e., very high molecular weight nucleic acids (e.g., 1–50 megadaltons)
Eluent in column: methanol