NUCLEODUR® is a fully synthetical type B silica (silica of 3rd generation) offering highly advanced physical properties:
- Totally spherical particle shape
- Outstanding surface microstructure
- High pressure stability
- Low metal content
NUCLEODUR® as a state-of-the-art silica is the ideal base material for modern HPLC phases. It is the result of MACHEREY-NAGEL's pioneering research in chromatography for almost 50 years.
NUCLEODUR® NH2 / NH2-RP
Aminopropyl modification for NP and RP separations, not endcapped, USP L8
Key features:
- Multi-mode columns (for RP, NP and IC)
- Stable against hydrolysis at low pH (working range pH 2–8)
- 100 % stable in water
- Suitable for LC/MS
- Widens scope of analytical HPLC into the polar range
Technical characteristics:
- Aminopropyl modified high purity silica
- Pore size 110 Å
- Particles sizes 3, 5 and 7 µm
- Carbon content 2.5 %
Recommended application:
- polar compounds under RP conditions (sugars, DNA bases), hydrocarbons under NP conditions
- Normal phase chromatography (NP) with hexane, dichloromethane or 2-propanol as mobile phase for polar compounds such as substituted anilines, esters, chlorinated pesticides
- Reversed phase chromatography (RP) of polar compounds in aqueous-organic eluent systems
- Ion exchange chromatography of anions and organic acids using conventional buffers and organic modifiers