The Bioptechs Delta T™ Culture Dish System is the only culture dish that provides heat to the contents of the dish without heating the stage of the microscope. It uses number 1.5 thickness cover glass bottomed dishes that have an electrically conductive, optically transparent coating of ITO on the underside. The coating is electrically powered to directly warm the contents of the dish. This is an exclusive technology to Bioptechs and provides features and capabilities no other system can match. An intelligent feedback loop passes an electrical current through a thin-film coating on the underside surface of the glass substrate on which the cells are grown. Heat is applied directly to the cells without the inefficiencies associated with peripheral heating by traditional peripherally heated culture dish warmers. Bioptechs exclusively offers opaque culture dishes which eliminates the unwanted ambient light background for fluorescence imaging. The controller features a real-time temperature display and fast learning curve to compensate for cooling due to surface evaporation while responding to temperature changes due to perfusion. There is also an alarmed protection circuit to safeguard the cells and an internal reference for the user adjustable calibration. The standard controller has a temperature range of ambient to 50°C.
Delta T™ dishes have 35mm O.D. and a 23mm central aperture. The peripheral region of the dish is tapered to reduce the dead-volume and the height of the dish is 6mm to allow better access for micro-injection and micromanipulation. The dishes are a hybrid of polystyrene plastic and Desag 263 glass. The outer structure of the dish is available in opaque black or clear and come with a clear 0.5mm or No. 1.5 glass coverslip bottom bonded to it. The dishes are also available in a plain glass unheated version.It accommodates a wide variety of specimen types including adherent cells, suspended cells, tissue, and artificial membranes. This system is ideal for multi-user facilities or those that don’t need a flow cell but are undecided about what type of chamber system is required. The Delta T™ has an assortment of convenient accessories that expand its capabilities. The most often used accessories are the Heated Lid, Coverglass Lid, and Culture Cylinders. Short of needing a defined flow for perfusion, the Delta T™ is the most convenient, accurate, means of micro-environmental control for a microscope.
Features
- Easily adapted to a wide variety of specimen types from monolayered adherent cells to brain slice and tissue preps
- Low mass to thermo-regulate as opposed to conventional stage heaters
- Plate, incubate, and observe without the need to transfer your cells
- Fast thermal recovery after perfusion (within seconds)
- Compatible with inverted and upright microscope stands (Stage Adapters)
- Coverglass bottom for optimum optical compatibility
- No need for warm air blowers or stage heaters
- Direct first-surface heating to your cells
- Can also be cooled below ambient
- Perfusion available (Perfusion Configuration)
- No preheating
Ideal for first time users and multi-user facilities. Bioptechs has combined the Delta T with the three most commonly used accessories to get you up and running as fast and trouble free as possible at a reduced price.The Bioptechs starter set will immediately give you the micro-environment base for your cells to start high end time lapse microscopy with the ease of disposable culture dishes, and the versatility to image a wide variety of cell and tissue cultures directly out of the box.
The starter set includes:
- (1) Delta T™ Controller
- (1) 10pk of Delta T™ Dishes 0.17mm thick (clear)
- (1) Coverglass Lid
- (1) Heated Lid with CO2 port
- (1) Culture Cylinder Pack, (1) 4mm, (1) 6mm, (1) 8mm, (1) 10mm
- (1) Standard Stage Adapter