This kit is designed to teach 8 groups of four students the principles of plasmid DNA purification by minipreps.
The kit contains the reagents to allow students to:
- Collect p-Amylase-transformed bacteria from an overnight culture
- Resuspend bacteria, lyse and isolate the p-Amylase plasmid by removing proteins and genomic DNA
- Bind the plasmid DNA to a spin column and wash
- Elute the plasmid DNA from column
- Measure the amount of purified pAmylase plasmid DNA with a unique DNA assay
Features
- For 8 groups of 4 students
- Requires high speed micro-centrifuge, micropipettes and tips
Applications
- HS-LS1: From Molecules to Organisms: Structures and Processes
- LS1.A: Structure and Function