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The rAmylase Project: Restriction Digestion to Verify the pAmylase Plasmid Kit

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This kit is designed to teach 8 groups of four students the principles of restriction digestion and agarose electrophoresis. The kit contains the reagents to allow students to:

  • Set up restriction digests of the p-Amylase plasmid with two enzymes
  • Set up positive and negative controls for the restriction digestion
  • Load and separate DNA samples on an agarose gel
  • Visualize digests of the plasmid on an agarose gel
  • Construct the restriction digestion map of the plasmid



  • For 8 groups of 4 students 
  • Requires agarose electrophoresis equipment including UV transilluminator (light box)
  • Micropipettes and tips



  • HS-LS1: From Molecules to Organisms: Structures and Processes
  • LS1.A: Structure and Function



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The rAmylase Project: Restriction Digestion to Verify the pAmylase Plasmid, 8 x 4 student groups
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