Restriction enzymes are DNA-cutting enzymes found in bacteria and as they cut within the molecule, are often called restriction endonucleases. A restriction enzyme recognizes and cuts DNA only at a particular unique sequence of nucleotides, allowing for restriction sites to be mapped.
The DNA Restriction Digestion Analysis kit demonstrates the specificity of restriction enzymes and their need for specific buffers. An extension of this allows students to understand the crucial role restriction enzymes play in molecular cloning and the analysis of genomic and recombinant DNA.
Supplied with components needed for hands-on experimentation for six workstations of 4-5 students or 24-30 students. Supplied with Teacher’s Guide and separate Student’s Guides.
Applications
- Supplied with multiple restriction enzymes.
- Learn about restriction enzyme specificity.
- Teaches the role or restriction enzymes in molecular cloning.