Gly-Phe-AFC is a fluorogenic, cell-permeant peptide substrate, used in Cytotoxicity testing. In vitro cytotoxicity testing has become an integral aspect of drug discovery because it is a convenient, costeffective, and predictive means of characterizing the toxic potential of new chemical entities.
Gly-Phe-AFC is a fluorogenic, cell-permeant peptide substrate use to measure number of live cells in cytotoxic assay. It enters intact cells where it is cleaved to generate a fluorescent signal proportional to the number of living cells. This live-cell protease activity marker becomes inactive upon loss of membrane integrity and leakage into the surrounding culture medium. It is also used as substrate for measuring Dipeptidyl peptidase1 (DPP1) activity. DPP1 is a lysosomal cysteinyl protease of the papain family involved in the intracellular degradation of proteins.
Key Applications: Substrate in Cytotoxicity Assay, Substrate in measurement of Dipeptidyl peptidase1 activity
Application Areas: Cell Migration Analysis; Cell Signaling Analysis
Research Areas: Calcium Regulation; ECM Remodeling; Cell Cycle; Transcription Regulation; Apoptosis Related Factors
Product Type: Proteins, Enzymes & Peptides
Protein or Enzyme Type: Activators, Inhibitors & Substrates
Presentation: White powder
Format: Powder
Detection Method: Chromatography: BAW: 4:1:1, Single Spot; 'H NMR: All functional groups present
NOTES: Amino acid Sequence: Gly-Phe-AFC·TFA GF-AFC·TFA
Solubility: 10mM solution in DMF or DMSO
Storage & Handling: Keep cool and dry