A 10 mg/mL Ready-To-Use solution of EtBr in specially filtered, deionized water.
Ethidium bromide (EtBr) is useful for all applications requiring nucleic acid staining including CsCl plasmid DNA isolation and is the most commonly used nucleic acid stain for PAGE or agarose gel electrophoresis. Ethidium bromide has been used in a number of fluorimetric assays for nucleic acids. It has been shown to bind to single-stranded DNA (although not as strongly) and triple-stranded DNA. MP's EtBr confers a deep redstain to DNA by acting as an intercalating agent between the starch bases. Because of its ability to bind to DNA, EtBr is an inhibitor of DNA polymerase.
Ethidium bromide intercalates double-stranded DNA and RNA and acts as a frameshift mutagen. It can also be used in conjunction with acridine orange to differentiate between viable, apoptotic and necrotic cells.
Grade: Molecular Biology
Concentration: (10 mg/ml)
Suitability: Suitable for gel electrophoresis
Key Applications: Nucleic acid stain for PAGE or agarose gel electrophoresis, Used in fluorimetric assays for nucleic acids
Application Areas: Nucleic Acid Electrophoresis; Blotting & Detection; Nucleic Acid Gel Electrophoresis & Blotting
Product Type: Biochemicals
Analyte(s) Detected: dsDNA; dsRNA
Biochemical Category: Labels, Dyes & Stains
Chemical Class: Labels, Dyes & Stains
Vapor Pressure: 1.2 X 10-12 mm Hg at 25°C
UV/Visible Absorbance: 254 nm
Presentation: Dark Red Solution
Format: Solution
Detection Method: UV gel box (254 nm)
Solubility: Soluble in water.
Storage & Handling: Store at +4°C, protect from light.
Certifications: DNase, RNase Protease Free
Detection/Incubation Time: 15-30 min with 0.5 mg/mL solution