Synonym(s): PDL Solution
CAS No.: 25988-63-0
Application: Recommended as a cell culture substratum when using 0.5-áml of a 0.1-ámg/ml solution to coat 25-ácm. Optimal conditions for attachment must be determined for each cell line and application.
General description: Poly-lysine polymers are useful molecules to prepare surfaces for cell attachment. Poly-lysine polymers are available in L and D conformations. Poly-L-lysine polymers can be digested by some cell types, whereas poly-D-lysines are stable. Poly-lysine polymers from 70 to 300 kDa provide sufficient attachment site density for good cell adherence. However, higher molecular weight poly-lysine polymers are more viscous and difficult to work with.
The choice of poly-lysine polymer for a specific cell type and application should account for the susceptibility of the polymer to digestion, the required attachment site density and the difficulty in handling.
Other Notes: This poly-L-lysine is relatively high density which provides good binding characteristics, but it is more viscous and difficult to handle. It may be degraded by some cell types. In that case a poly-D-lysine should be chosen.
Storage: 2-8°C
Empirical Formula (Hill Notation): C4H6O