The total protein is measured using the Biuret procedure, with bovine albumin as the standard. The product gives a spectrum of normal serum precipitin arcs when tested by immunoelectrophoresis with anti-horse serum.
The serum is delipidated, dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.05% sodium azide, filtered through a 0.22 μm filter, vialed and lyophilized.
Whole horse serum is suitable for use as a serum protein control in immunoelectrophoresis, Ouchterlony immunodiffusion, and other immunoprecipitation techniques. It may also be used as a blocking agent in fluorescence and enzyme immunoassays.
Product is whole horse serum and buffer salts.
Reconstitution: Reconstitute product with 2.0 mL of deionized or distilled water. Gentle swirling may be used to speed rehydration. Avoid vigorous shaking of the reconstituted material.
Concentration: Total Protein: 63.5 mg/mL
Application Areas: Immunoassays
Product Type: Culture Media
Culture Media Type: Animal Serum
Format: Lyophilized
Formulation: Delipidated, Lyophilized from PBS with azide
Storage & Handling: Store product at 2-8°C. Store reconstituted material no longer than 4 weeks at 2-8°C. For long-term storage, aliquot and freeze reconstituted product at -20°C or lower.