Product is the lyophilized powder of goat antiserum to swine serum and buffer salts.
Goat antiserum to swine serum (forensic) is used in immunostaining of cell suspensions, acetone-fixed frozen tissue sections, and formalin-fixed, paraffin-embedded tissue sections. This product is intended for use as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis, capillary tube, and immunodiffusion techniques.
The total protein is measured using the Biuret procedure, with bovine albumin as the standard.
The antiserum is prepared by immunizing goats with swine serum; antiserum is delipidated and made specific by immunoadsorption with soluble antigens or solid-phase immunoadsorption where appropriate. The resulting antiserum is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.05% sodium azide, adjusted to standard titer, filtered through a 0.22 μm filter, vialed and lyophilized.
Reconstitution: Reconstitute product with 2.0 mL of deionized or distilled water. Gentle swirling may be used to speed rehydration. Avoid vigorous shaking of the reconstituted material. If a small amount of precipitate is present, or if preparation appears cloudy, clarify by centrifugation or filtration.
Concentration: Total Protein: 59.9 mg/mL
Application Areas: Immunoassays
Product Type: Antibodies
Antibody Type: Primary Antibodies
Antibody Category: Polyclonal
Species Reactivity: Deer
Formulation: Lyophilized PBS with 0.05% Sodium Azide
Storage & Handling: Store product at 2-8°C. Store reconstituted material no longer than 4 weeks at 2-8°C. For long-term storage, aliquot and freeze reconstituted product at -20°C or lower