Sodium phosphate is a reagent with very high buffering capacity that is widely used in molecular biology, biochemistry, and chromatography. Sodium phosphate occurs in several forms: monobasic (NaH2PO4), dibasic (Na2HPO4), and tribasic (Na3PO4). Most neutral sodium phosphate buffer solutions consist of mixtures of the monobasic and dibasic forms to varying degrees, depending on the desired pH.
Sodium Phosphate Monobasic, Monohydrate is used as sequestrant, emulsifier and buffer in foods; as mordant in dyeing; for weighting silk in tanning; in manufacturing of enamels, ceramics, detergents, boiler compounds; as fireproofing agent; in soldering and brazing instead of borax; as reagent and buffer in analytical chemistry; cathartic; laxative. A table for preparation of 0.1 M sodium phosphate buffer at 25°C using various proportions of sodium phosphate monobasic and sodium phosphate dibasic has been published. A study of the effect of freeze-thaw storage cycles on proteins in sodium phosphate and potassium phosphate buffer solutions has been reported. The effect of 5 mM sodium phosphate on the efficacy of electrospray ionization (ESI) ion mobility spectrometry (IMS) analysis has been evaluated. A protocol for the purification of pyrogen-free mouse IgG1 monoclonal antibodies which uses 10 mM sodium phosphate (pH 7.4) has been published. An ion-pairing HPLC method for the analysis of 5-aminosalicylic acid has been reported. A TLC method for separation of nucleotide sugars in the study of glycosyltransferase activity has been published.
Grade: USP
Assay: 98.0-103.0 %
Key Applications: Biological Buffers, Chemical Synthesis
Product Type: Inorganics
Chemical Class: Salts
Melting Point: 214°C(Lit.)
Presentation: White Powder
Format: Powder
pH: 4.1 - 4.5 (5% aq soln)
Heavy Metals: ≤0.002%
Insoluble Matter: ≤0.2%
Water Content: 10 - 15%
NOTES: Chloride ≤0.014% ; Sulfate ≤0.15%; Arsenic ≤8 ppm
Solubility: Soluble in water (100 mg/mL), yielding a clear, colorless solution.
Storage & Handling: Room Temperature, Desiccate