Potassium ferrocyanide trihydrate is a salt of the Fe(CN)64- ferrocyanide ion. Ferrocyanide is a reducing iron species, being oxidized from ferrous Fe(II) to ferric Fe(III).
Potassium ferrocyanide is used to determine the concentration of potassium permanganate, a compound often used in titrations based on redox reactions. Potassium ferrocyanide is used in a mixture with potassium ferricyanide and phosphate buffered solution (PBS) to provide a buffer for X-Gal, which is used to cleave Beta-galactosidase, giving a bright blue visualization where an antibody (or other molecule), conjugated to Beta-gal, has bonded to its target.
The ferrocyanide species is membrane impermeable and can be used to assess redox activity of membrane-bound structures and mechanisms of electron transport across membranes. This approach was employed to demonstrate that Potassium ferrocyanide performs reducing activation of membrane-bound dopamine β-monooxygenase from the exterior face of adrenal vesicles, providing elucidation of native reduction-activation mechanisms for dopamine β-monooxygenase regulation.
Grade: ACS Reagent Grade
Assay: 98.5 - 102.0%
Application Areas: Molecular Biology
Product Type: Biochemicals
Density: 7.87 g/cm3 at 25°C (Lit.)
Melting Point: 70°C(lit.)
Vapor Pressure: ca. .000001 hPa at 25°C (Lit.)
Presentation: Yellow Powder
Format: Powder
Insoluble Matter: ≤0.005%
NOTES: Chloride ≤0.01%
Storage & Handling: Store at Room Temperature (15-30°C)