Application: The selection of plasmids in yeast is based on the use of auxotrophic mutant strains that cannot grow without a specific media component (an amino acid, purine or pyrimidine). Transformation with a plasmid containing the mutated gene enables the transformant to grow on a medium lacking the required component.1 Sigma′s Yeast Synthetic Drop-Out Media Supplements create a richer medium for better yield and growth rate, and increase the probability of successful transformations when screening libraries or performing gene knock-outs.
Components: The supplements contain all of the following components except as indicated:
Amino acids: All standard amino acids except for leucine and tryptophan. All the amino acids are present at a concentration of 76 mg/L.
Other nutrients: Adenine (18 mg/L), inositol (76 mg/L), p-aminobenzoic acid (8 mg/L), uracil (76 mg/L).
Quantity: 1.54 g will supplement 1 liter of medium.
Other Notes: Mixtures of amino acids and other nutrients to be added to Yeast Nitrogen Base Without Amino Acids.