Synonym: 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol
CAS Number: 77-86-1
Linear Formula: NH2C(CH2OH)3
Molecular Weight: 121.14
Beilstein Registry Number: 741883
EC Number: 201-064-4
MDL Number: MFCD00004679
PubChem Substance ID: 24889854
Other Notes
Running buffer component in non-denaturing agarose gel electrophoresis; In non-denaturing and urea-denaturing polyacrylamide gel electrophoresis; DNA digest analysis with capillary electrophoresis.
The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution. For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Application
Trizma is used in the formulation of buffer solutions in the pH range between 7.5 and 8.5. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification. Trizma based buffers are also in column chromatography and in gel electrophoresis. Trizma base is used as a general reagent for the preparation of all types of Tris buffers.
Legal Information
Trizma is a registered trademark of Sigma-Aldrich Co. LLC
Grade: for molecular biology
Description: aminopeptidase substrate
Product Line: BioUltra
Assay: ≥99.8% (T)
Impurities: DNases, none detected; RNases, none detected; insoluble matter, passes filter test; phosphatases, none detected; proteases, none detected
Ign. Residue: ≤0.01% (as SO4)
Loss: ≤0.5% loss on drying, 110 °C
pH: 10.5-12.0 (25 °C, 4 M in H2O)
Useful pH Range: 7 - 9
pKa (25°C): 8.1
BP: 219-220°C/10 mmHg(lit.)
MP: 167-172°C(lit.); 168-172°C
Solubility: H2O: soluble4 M at 20°C, clear, colorless
Anion Traces: chloride (Cl-): ≤20 mg/kg; sulfate (SO42-): ≤5 mg/kg
Cation Traces: Al: ≤5 mg/kg; As: ≤0.1 mg/kg; Ba: ≤5 mg/kg; Bi: ≤5 mg/kg; Ca: ≤10 mg/kg; Cd: ≤5 mg/kg; Co: ≤5 mg/kg; Cr: ≤5 mg/kg; Cu: ≤5 mg/kg; Fe: ≤5 mg/kg; K: ≤50 mg/kg; Li: ≤5 mg/kg; Mg: ≤5 mg/kg; Mn: ≤5 mg/kg; Mo: ≤5 mg/kg; Na: ≤50 mg/kg; Ni: ≤5 mg/kg; Pb: ≤5 mg/kg; Sr: ≤5 mg/kg; Zn: ≤5 mg/kg;
λ: 4 M in H2O
UV Absorption: λ: 260 nm Amax: 0.10; λ: 280 nm Amax: 0.08
Suitability: in accordance for luminescence