Synonym: 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trizma® base, Trometamol, amino-2-(hydroxymethyl)-1,3-propanediol, 2-
CAS Number: 77-86-1
Linear Formula: NH2C(CH2OH)3
Molecular Weight: 121.14
Beilstein Registry Number: 741883
MDL Number: MFCD00004679
Other Notes
For life science research only. Not for use in diagnostic procedures.
The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Quality
Contaminants: <0.001% PK and aldolase, each, <0.01% GOT, GPT, MDH and myokinase, each
Legal Information
Trizma is a registered trademark of Sigma-Aldrich Co. LLC
Description: aminopeptidase substrate
Assay: ≥99.9% (titration)
Packaging: pkg of 1 kg (10708976001); pkg of 5 kg (11814273001)
Mfr. No.: Roche
Useful pH range: 7 - 9
pKa (25°C): 8.1
BP: 219-220 °C/10 mmHg(lit.)
MP: 167-172 °C(lit.)
Absorption: A100mg/mL/300 nm ≤0.015
Shipped In: ambient
Storage Temp.: 20-25°C