Synonym(s): SBTI
CAS No.: 9035-81-8
Application: After trypsinizing cells, resuspend cells in 1 mL trypsin inhibitor solution (1mg/ml) for every mL of trypsin solution used for dissociation. Centrifuge the cell suspension at 1000 rpm for 5 minutes. A cell pellet should form. Remove as much of the trypsin inhibitor solution as possible and resuspend the pellet in serum-free medium. Culture cells as desired.
General description: Soybean trypsin inhibitor exhibits a high degree of sequence homology with cathepsin D inhibitor from potatoes.1
Special Activity: activity: (One mg will inhibit 1.0-3.0 mg of trypsin with activity of approx. 10,000 BAEE units per mg protein.)
Analysis Note: Protein determined by biuret.
Preparation Note: Trypsin inhibitor is soluble in water and phosphate buffers at concentrations of 10 mg/ml or higher. Solutions at higher concentrations may be hazy and have a yellow to amber color.
Other Notes: View more information on Trypsin Inhibitor.
Storage: 2-8°C
EC No.: 232-906-9
Biochem/physiol Actions: Soybean trypsin inhibitor inhibits trypsin and to a lesser extent chymotrypsin and plasmin. It forms a 1:1 stoichiometric complex with trypsin. Upon formation of this complex, trypsin may cleave a single arginine-isoleucine bond in the inhibitor. Dissociation of this complex may yield the modified form or the native inhibitor. At the optimal pH for trypsin binding (pH 8.0), the association constant is ≥ 10x108.
Unit Definition: One trypsin unit = ΔA253 of 0.001 per minute with N−α-benzoyl-L-arginine ethyl ester (BAEE) as substrate at pH-á7.6 at 25-á°C. Reaction volume = 3.2 mL (1 cm light path).
Empirical Formula (Hill Notation): C6H10O5