Synonyms: 2-Amino-2-(hydroxymethyl)-1,3-propanediol; THAM; Tris base; Tris(hydroxymethyl)aminomethane; Trometamol
Formula: NH2C(CH2OH)3
Formula Weight: 121.14
CAS No.: 77-86-1
Purity: ≥99.9% (titration)
Other Notes: The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Legal Information: Trizma is a registered trademark of Sigma-Aldrich Co. LLC
Abs.: A1M/280, H2O ≤0.020
Anion Traces: chloride (Cl-): ≤0.005%
Assay: ≥99.9% (titration)
BP: 219-220°C/10 mmHg(lit.)
Cation Traces: Ba: ≤0.0005%
Description: aminopeptidase substrate
Ign. Residue (900°C): ≤0.01% (as SO4)
Impurities: ≤0.005% Insoluble matter
Loss: ≤0.5% loss on drying, 110°C
MP: 167-172°C(lit.)
pH: 10.5-12.0 (1 M in H2O)
pKa (25°C): 8.1
Product Line: BioXtra
Quality Level: ELITE
Solubility: H2O: soluble1 M, clear, colorless
Useful pH Range: 7 - 9