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MP Biomedicals

Tween®80

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Tweens® are a series of nonionic surfactants derived from sorbitan esters. They are soluble or dispersible in water but differ widely in organic and oil solubilities. Used as oil-in-water emulsifiers in pharmaceuticals, cosmetics, cleaning compounds, etc.

Tween® 80 has been widely used in biochemical applications including: solubilizing proteins, isolating nuclei from cells in culture selective protein extraction growing of tubercule bacilli , and emulsifying and dispersing substances in medicinal and food products. It has little or no activity as an anti-bacterial agent. It has been shown to have an adverse effect on the antibacterial effect of methyl paraben and related compounds. Non-ionic detergent used for selective protein extraction and isolation of nuclei from mammalian cell lines.

Grade: Cell Culture Reagent
Concentration: ~75% oleic acid
Suitability: Suitable for cell culture
Key Applications: Selective protein extraction, Isolation of nuclei from mammalian cell lines
Application Areas: Cell Biology & Analysis
Product Type: Biochemicals
Biochemical Category: Surfactants
Chemical Class: Surfactants
Density: 1.08 g/mL
Specific Gravity: 1.06 - 1.09
Viscosity: 300 - 500 cps
Boiling Point: 100 °C (Lit.)
Presentation: Liquid
Format: Liquid
Critical Micelle Concentration (CMC): 0.012 mM (13-15 mg/L)
Residue on ignition: <0.25%
Water Content: ≤3.0%
NOTES: Saponification Value: 45-55; Acid no:0-2.0; Hydroxyl No. 65-80
Solubility: Soluble/miscible in water to give a clear yellow solution; miscible with alcohol, dioxane, and ethyl acetate; practically insoluble in liquid paraffin and fixed oils (such as mineral oil).
Sterilization of Solutions: Autoclaving of solutions is not recommended. Sterile filtering is suggested with a 0.22 micron filter. Tween® may need to be warmed to about 40 °C and alternated with portions of hot distilled water while being poured through the filter.
Storage & Handling: Store at Room Temperature (15-30 °C).

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Product Detail
Thomas No.
C833V16
Mfr. No.
02194725.1
Pkg. Size
100mL
CAS #
9005-65-6
Description
Tween®80 100mL

Grade
Cell Culture Reagent
list price/quantity total
$0.00
Thomas No.
C833V17
Mfr. No.
0219472504
Pkg. Size
4L
CAS #
9005-65-6
Description
Tween®80 4L

Grade
Cell Culture Reagent
list price/quantity total
$0.00
$0.00 (0 Items)

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