The SygRNA modified synthetic RNA system recruits WT SpCas9, eSpCas9, dCas9, or Cas9D10A to specific genomic targets. 2' O-methyl and phosphothioate modifications have been incorporated to enhance the stability and performance of the synthetic RNA. The modified tracrRNA (trans-activating crRNA) binds to a crRNA (CRISPR RNA) and the resulting crRNA-tracrRNA complex guides SpCas9 and SpCas9 variant proteins to crRNA-specified sites in the genome. SygRNA modified synthetic tracrRNA and crRNAs are optimized for use with a variety of Cas9 expressing cell lines, Cas9 proteins, or Cas9 mRNAs and are compatible with a delivery methods such as microinjection, lipofection, and electroporation.
Application: Functional Genomics/Target Validation/Transgenics
•Creation of gene knockouts in multiple cell lines
•Complete knockout of genes not amenable to RNAi
•Creation of knock-in cell lines with promoters, fusion tags, or reporters integrated into endogenous genes
•Generation of transgenic animals via embryo microinjection or electroporation
Legal Information: SygRNA is a registered trademark of Sigma-Aldrich Co. LLC