Superoxide Dismutase from bovine erythrocytes BioUltra, lyophilized powder, =4,500 units/mg protein, =97% (SDS-PAGE)

Synonym: SOD, Superoxide: superoxide oxidoreductase

CAS Number: 9054-89-1

EC Number: 232-943-0

Enzyme Commission (EC) Number: 1.15.1.1

MDL Number: MFCD00132404

Biochem/physiol Actions
Catalyzes the dismutation of superoxide radicals to hydrogen peroxide and molecular oxygen. Plays a critical role in the defense of cells against the toxic effects of oxygen radicals. Competes with nitric oxide (NO) for superoxide anion (which reacts with NO to form peroxynitrite), thereby SOD promotes the activity of NO. SOD has also been shown to suppress apoptosis in cultured rat ovarian follicles, neural cell lines, and transgenic mice.

General Description
Superoxide dismutases are a group of low molecular weight metalloproteins present in all aerobic cells of plants, animals and micro-organisms. They provide protection against damaging reactions with the superoxide radical anion (O2-) by catalyzing its disproportionation into oxygen and hydrogen peroxide.

Unit Definition
One unit will inhibit reduction of cytochrome c by 50% in a coupled system with xanthine oxidase at pH 7.8 at 25 °C in a 3.0 mL reaction volume. Xanthine oxidase concentration should produce an initial ΔA550 of 0.025 ± 0.005 per min.

Application
Superoxide Dismutase from bovine erythrocytes has been used in a study to assess activity parameters in adjuvant-induced arthritis. Superoxide Dismutase from bovine erythrocytes has also been used in a study to simplify the process of purifying superoxide dismutases using charge-controlled hydrophobic chromatography on 10-carboxydecyl Sepharose.